Feeling a bit flat
There is a wonderfully helpful spirit in the scientific community, especially when it comes to sharing reagents and tools. If one lab develops something handy and publishes it in a paper, interested readers need only to drop an email to the lab head and request a sample for their own experiments. (In fact, most journals deem such assistance mandatory, though I don’t think your average researcher needs threats as an incentive to share the love.)
While sometimes such requests fall on deaf ears, in my experience, being ignored or fobbed off is rare. So if everyone is all friendly and touchy-feely about providing stuff promptly, how then can we measure relative kindness?
Clearly, some help is more helpful than others. When it comes to plasmid DNA, it is relatively straightforward for a researcher to make copies from a tiny, forensic amount of the original. Because of this, it’s become trendy in recent years to send out DNA, not in a goodly amount in a tube, but as spots dried onto a piece of filter paper. This spot can be rehydrated and zapped into E. coli bacteria, which will create copies of the plasmid which you can then liberate from the bugs in large quantities.
No big deal, right? But it does take at least two days, and a few hours of your life manipulating bugs, plates and columns at your bench. Whereas if someone sends you a lovely big wodge of DNA, and you only want to do an experiment a few times, you’d be able to perform it straightaway without faffing around with the cloning step.
Earlier this week, I requested a plasmid from a very big, very famous American lab. I was charmed that the professor answered personally, on the same day he received the email, and that he immediately instructed his post-doc to send it out. I was further charmed by the friendliness of the post-doc, who emailed to say he’d sent it Fedex that very same day.
It had not escaped my notice, though, that if the plasmid arrived today, and there was a goodly amount, I could perform my experiment this afternoon and have slides ready on Monday, which in turn could be imaged and analyzed before I went on holiday that coming weekend – giving me a significant edge on my paper revision deadline. I wouldn’t dare have asked for more than a dried spot, but I was sort of hoping the post-doc would sense my desperate, fellow-post-doc-angst vibes across the many miles between us.
And so it was that the Fedex package arrived. And I knew immediately how the DNA had been sent — the package was flat as a pancake. I fondled it optimistically, but there was definitely no tube in there, not even one of those weeny PCR-sized tubes.
Ah well, I thought philosophically as I roused the bugs from their dormant sleep in the -70 freezer. It’s definitely possible to be too greedy. The lab in question probably sends out hundreds of plasmids a week, and it’s just not practical to feed the five thousand. In fact, the post-doc was doing me a favor: I have way too much on on Monday, and another experiment probably would have tipped the balance.
Besides, I like bacterial work: it reminds me of my lost youth as a bona fide microbiologist.
So let’s hear it for the kindness of strangers, something that definitely makes the scientific world go round.
Somewhat surprisingly, many of my colleagues on your side of the pond not only request dried DNA rather than ready-to-go DNA plasmids, but they actually also request that the material be sent by royal mail rathen than courier. Apparently, they either fear customs delays or want to save money.
Any insight from your angle?
The tube isn’t going to change the weight appreciably if it’s small and filled with 20-30 uL of liquid, is it? We don’t have customs problems with DNA – only Drosophila, which routinely arrive dead from the States after their extended holiday at Heathrow Airport.
Actually, I’ve just thought – the lab head would almost certainly prefer the filter paper – it’s not like he has to actually prep the damned thing! I think all postdocs and PhDs are going to want the liquid, because time is definitely money.
It’s actually easier to send and receive filter paper—it just looks like a letter.
Especially in Australia, we never received tubes–it always got held up in customs and they asked for a release fee (not to mention reams of paperwork). Filter paper just sailed through.
Uh, that was me, btw.
Well, if it isn’t a customs issue then the PI must be trying to save a few quid/euros–because the request for regular mail posting would generally come when I would ask if they want to provide a Fedex airbill number.
This is why I always request my own reagents…;-)
I must admit, if I’ve only got a miniprep of something, I’ll spot it out. Way easier and can be put in an envelope. But if it’s someone I know personally or has come to me personally rather than my PI I’m much more inclined to send a tube. I guess there’s not harm in asking for one, it’s so nice to not have to transform, and of course it does cost time and money to do it.
[…] In sum, this is not going to be the insightful blog post of your dreams. Instead, I leave you with the following tantalizing picture as a place-holder – one of the images from one of my backup experiments, expressing a construct I received recently. […]
Not plasmids, but my best ‘customs horror story’ was when I was on Sabbatical in the late 90s and sent some precious aliquots of polyclonal antibody, on dry ice, copiously labelled “on dry ice’ ‘urgent’ ‘scientific reagents’ etc etc, express delivery from myself in Manchester (Xmas break) to myself at the NIH in Bethesda (Sabbatical location).
Flying back to DC the next day, I expected the antibody parcel to arrive in a day or so. About a week later, I got a phone call from a Customs / FDA (can’t remember which) warehouse somewhere in the Virgina boonies, telling me the parcel had been impounded, opened, and left to sit around in their facility since it did NOT have, written prominently on it, ‘contains no bovine material’. Needless to say it had long since defrosted.
Oh, man. I once spent a week preparing a protein from chicken gizzards (as indeed I spent most of my life as a grad student) and sent it to our collaborators in Memphis… only for it to arrive as everyone in customs had gone home for the weekend.
Too long in the presence of the King…
I, on the other hand, once took live C. elegans cultures across a major international border (and a rather large ocean), in my pocket to avoid them being X-rayed.
Hang on, I think I hear Canada Customs at my door… *muffled banging and sirens*