Yes, it really *is* that simple
I’ve been showing a newbie how to work with bacteria, and today her transformation plates were sprinkled with dozens of lovely colonies. I described to her how she should inoculate a single colony into antibiotic broth and left her to it.
After lunch, she came to my desk with a worried crease to her brow.
“What it it?” I said.
“The colony,” she said. “Are you sure it’s going to work?”
“It’ll work,” I said.
“But…I couldn’t actually see anything on the tip before I put it into the broth.”
“It’ll work.”
“Are you sure there was anything on the tip?”
“Show me the plate,” I said. She did so, and pointed out the colony she had selected, now smeared into a tiny linear smudge by the action of the sterile plastic yellow tip she had used to collect it.
“It will work,” I said. “It’s all down to the wonders of exponential growth.”
She didn’t look entirely satisfied, but come tomorrow, I’m sure she’ll be convinced by 100 mL of frothy bacterial goodness.
ahh… the goodness of single colony inoculation! It’s hard to remember sometimes that there are soooooo many bacteria in one colony. (maybe it’s just to keep sane I still can forget? 😉 )
Hope it all goes well tomorrow!
To be fair, I had the same newbie experience a few weeks ago, scraping a tiny bit of yeast into some buffer and not really believing I’d get enough genomic DNA for PCR – but of course I had tons. It’s all a question of faith.
…and??
Froth city.
Was she impressed?
First time I did this (during A-level) me and my friends were so worried that there was nothing on the spreaders that we put about six loads of them on the plates. We ended up with massively overgrown plates and an unsurprising lack of single colonies!
Learning to have faith in the microscopic is one of the first hurdles of the new biologist…