If you’re like me, you might sometimes wonder what would happen if you were to omit particular steps in an experimental protocol. I’m the sort who religiously follows instructions, even when I suspect they’re not that important, so I don’t often have the chance to find out.

Not so our poor rotating graduate student. This afternoon, he discovered  exactly what transpires when you don’t wet your membrane in methanol prior to equilibrating in buffer and performing a Western blot.

Utter carnage.

Don't try this at home, kids